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	Provedor de dados OAK (2) Autor Shirafuji, Hiroaki (2) Xuan, Xuenan (2) Fujisaki, Kozo (1) Fukumoto, Shinya (1) Hirata, Haruyuki (1) Huang, Xiaohong (1) Liao, Min (1) Otsuka, Haruki (1) Suzuki, Hiroshi (1) Takashima, Yasuhiro (1) Zhang, Guohong (1) Mais... Palavra-chave C. parvum (1) Cross-reactive antigen (1) DNA vaccine (1) Fc (1) Heat-shock protein (1) Interferon-8 (1) Neospora caninum (1) Protein disulfide isomerase (1) Ribosomal protein 1 (1) Toxoplasma gondii (1) Mais... Ano 2005 (2) País Japan (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice OAK Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki. To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility of the plasmid pCX-p23Fc as a DNA vaccine... Palavras-chave: C. parvum; DNA vaccine; Fc; Interferon-8. Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/147 Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii OAK Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo. Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14... Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1. Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816 Registros recuperados: 2 Primeira ... 1 ... Última

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